When we search for a direction, we have intention to use that directional information to move towards it. Similarly, these polarly localized proteins direct cellular organelle, such as nucleus. It is an ideal scenario for asymmetric cell division, where nucleus needs to move away from the center of the cell.
This directional nuclear movement is facilitated by the usual suspect, cytoskeleton. In cellular context, cytoskeletons act as train track for nucleus movement. Plant cell biologists took advantage of many elegant cell types to combine cell polarity, cytoskeleton-dependent nuclear movement, and asymmetric cell division.
This directional nuclear movement is facilitated by the usual suspect, cytoskeleton. In cellular context, cytoskeletons act as train track for nucleus movement. Plant cell biologists took advantage of many elegant cell types to combine cell polarity, cytoskeleton-dependent nuclear movement, and asymmetric cell division.
The next interesting question is: Who is the master regulator in this cellular process? Nucleus decides the future cell division site? Or nucleus follows the instructions to position towards the already decided future cell division site?
Classic experiments utilized drug treatment (inhibiting cellular cytoskeleton track) or external cues (centrifugation, light) tried to displace nucleus and found that the future cell division happens according to the misplaced nuclear position. But, these conditions, we not only displace nucleus, but also hampers the overall cellular activities.
In a recent study, Arif took advantage of the nucleus envelope proteins, which connect nucleus and cytoskeleton for their movement. A mutation in the nuclear envelope protein disturb the link between nucleus and cytoskeleton, without disturbing other cellular processes. Using long-term live cell imaging, Arif discovered that when nucleus fails to go to the destination, cell decides to divide according to the misplaced nuclear position. In the following figure, Janlo Robil summarizes this exciting discovery.
Classic experiments utilized drug treatment (inhibiting cellular cytoskeleton track) or external cues (centrifugation, light) tried to displace nucleus and found that the future cell division happens according to the misplaced nuclear position. But, these conditions, we not only displace nucleus, but also hampers the overall cellular activities.
In a recent study, Arif took advantage of the nucleus envelope proteins, which connect nucleus and cytoskeleton for their movement. A mutation in the nuclear envelope protein disturb the link between nucleus and cytoskeleton, without disturbing other cellular processes. Using long-term live cell imaging, Arif discovered that when nucleus fails to go to the destination, cell decides to divide according to the misplaced nuclear position. In the following figure, Janlo Robil summarizes this exciting discovery.
Nucleus decides the future cell division site?
We are at the very beginning of our understanding that nucleus decides the future cell division site. The pivotal role in nuclear movement is played by nuclear envelope proteins. We are trying to systematically dissect which nuclear envelope proteins are involved in nuclear movement prior to asymmetric cell division. To answer this questions, we are using subsidiary cell during stomatal development in maize and lateral root development in the Arabidopsis. In both cases, the nucleus requires to move prior to asymmetric cell division. We combine our expertise on plant physiology, genetics, molecular, and cell biology to tackle this question.
We are at the very beginning of our understanding that nucleus decides the future cell division site. The pivotal role in nuclear movement is played by nuclear envelope proteins. We are trying to systematically dissect which nuclear envelope proteins are involved in nuclear movement prior to asymmetric cell division. To answer this questions, we are using subsidiary cell during stomatal development in maize and lateral root development in the Arabidopsis. In both cases, the nucleus requires to move prior to asymmetric cell division. We combine our expertise on plant physiology, genetics, molecular, and cell biology to tackle this question.
Nuclear membrane proteins regulate the cell division?
Nucleus maintains intact double layer membranes. During the cell division, nuclear envelope breakdowns and at the end of the mitosis, the envelope reappears in the daughter cell nucleus. In the meantime, the nuclear envelope proteins are observed to co-localize with mitotic apparatus. We are interested to know the function of nuclear envelope proteins during the mitosis. To answer this questions, we use symmetric cell division using the leaf meristematic region of maize and root meristematic region of Arabidopsis. This project harness the power of genetic and cell biology tools to perform the experiments.
Nucleus maintains intact double layer membranes. During the cell division, nuclear envelope breakdowns and at the end of the mitosis, the envelope reappears in the daughter cell nucleus. In the meantime, the nuclear envelope proteins are observed to co-localize with mitotic apparatus. We are interested to know the function of nuclear envelope proteins during the mitosis. To answer this questions, we use symmetric cell division using the leaf meristematic region of maize and root meristematic region of Arabidopsis. This project harness the power of genetic and cell biology tools to perform the experiments.
In this project, we explore how plants build these barriers in root using cellular, molecular, and genetic tools. Furthermore, we are interested to find out the function of exodermis barrier in challenging climate and during domestication.